1939-8G2細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
ATCC Number： HB-8134?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
1939-8G2細(xì)胞**類(lèi)型： IgG1
Designations: 1939-8G2
Depositors: Univ. Texas System, Board of Regents
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:1939-8G2細(xì)胞 immunoglobulin; monoclonal antibody; against Treponema pallidum
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Comments: Animals were immunized with T. pallidum.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with T. phagedenis.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. 1939-8G2細(xì)胞To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3539: Kettman JR, Norgard MV. 1939-8G2細(xì)胞Hybrid cell lines producing monoclonal antibodies directed against Treponema. US Patent 4,514,498 dated Apr 30 1985
