786-O細胞

器官來源： 腎臟

運輸方式： 凍存運輸

數(shù)量： 大量

細胞形態(tài)： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

年限： 58 years

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-1932?

相關**： 其他**

786-O細胞Designations: 786-O [786-0]

Depositors: RD Williams

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: kidney

Disease: renal cell adenocarcinoma

Cellular Products: parathyroid hormone (PTH) like peptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 786-O細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 8

D16S539: 12

D5S818: 9

D7S820: 11,12

THO1: 6,9.3

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: hypertriploid; Y was present in 60% the cells examined

Age: 58 years

Gender: male

Ethnicity: 786-O細胞Caucasian

Comments: This line was derived from a primary clear cell adenocarcinoma.

The cells display both microvilli and desmosomes, and can be grown in soft agar.

The cells produce a PTH like peptides that is identical to peptides produced by breast and lung tumors.

The peptide has an N terminal sequence similar to PTH, has PTH like activity, and has a molecular weight of 6000 daltons.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

786-O細胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22648: Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528

22653: Williams RD, et al.786-O細胞 In vitro cultivation of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102

23304: Thiede MA, et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897