PANC-1細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

數(shù)量： 大量

年限： 56 years

器官來(lái)源： 胰腺

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1469?

相關(guān)**： 其他**

組織來(lái)源： duct

細(xì)胞形態(tài)： 上皮樣

PANC-1細(xì)胞Designations: PANC-1

Depositors: M Lieber

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: pancreas

Tissue: duct

Disease: epithelioid carcinoma

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Applications: transfection host

DNA Profile (STR): PANC-1細(xì)胞Amelogenin: X

CSF1PO: 10,12

D13S317: 11

D16S539: 11

D5S818: 11,13

D7S820: 8,10

THO1: 7,8

TPOX: 8,11

vWA: 15

Cytogenetic Analysis: Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%.

Isoenzymes: G6PD, B

Age: 56 years

Gender: male

Ethnicity: Caucasian

Comments: Growth is inhibited by 1 unit/ml L-asparaginase.

The cells will grow in soft agar.

PANC-1細(xì)胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Doubling Time: 52 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22850: Lieber M, et al. PANC-1細(xì)胞Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870

22859: Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903