HCT-15細胞

器官來源： 結腸

運輸方式： 凍存運輸

ATCC Number： CCL-225?

相關**： 大腸癌

細胞形態(tài)： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

年限： Dukes' type C

Designations: HCT-15

HCT-15細胞Depositors: DL Dexter

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 5.4 ng/10 exp6 cells/10 days; keratin

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Applications: HCT-15細胞transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,11

D16S539: 12,13

D5S818: 13

D7S820: 10,12

THO1: 7,9.3

TPOX: 8,11

vWA: 18,19

Cytogenetic Analysis: This is a quasidiploid human cell line with the modal number 46 occurring in 76% of cells (range = 41 to 47 for 50 metaphases).The rate of polyploidy was 5.1%. The karyotype of the line 46, XY, -8,-11, -17, t(8:17)(p23:q21), inv(11)(p15.3q13.1). The Y chromosome was slightly longer than N22, and had a large segment of heterochromatic, fluorescent distal q arms.

Isoenzymes: ES-D, 2

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1-2

PGM3, 1

Gender: male

HCT-15細胞Comments: Evidence from DNA fingerprinting indicates that this line and DLD-1 (ATCC CCL-221) are derived from the same individual; however, isoenzymology and cytogenetic data leave some doubt.

HCT-15 cells are CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: HCT-15細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22417: Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23033: Dexter DL, et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742

32266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

46971: Vermeulen SJ, et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040