SW620細(xì)胞

數(shù)量： 大量

ATCC Number： CCL-227?

相關(guān)**： 大腸癌

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來源： 人

器官來源： 結(jié)腸

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： Dukes' type C

Designations: SW620 [SW-620]

Depositors: A Leibovitz

SW620細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Derived from metastatic site: lymph node

Cellular Products: carcinoembryonic antigen (CEA) 0.15 ng/10 exp6 cells/10 days; transforming growth factor alpha; keratin; matrilysin

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Applications:SW620細(xì)胞 transfection host

Tumorigenic: Yes

Oncogene: myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -

Antigen Expression: blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 9,13

D5S818: 13

D7S820: 8,9

THO1: 8

TPOX: 11

vWA: 16

SW620細(xì)胞Cytogenetic Analysis: modal number = 50; range = 45 to 53, The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 2

PGM3, 1

Age: 51 years

Gender: male

Ethnicity: Caucasian

Comments: SW620 was isolated from the tissue of a 51-year-old Caucasian male (blood group A, Rh+) as was SW480 (ATCC CCL-228).

A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass.

SW620 was initiated by A. Leibovitz, et al., from a lymph node in the same manner as was the primary adenocarcinoma from which SW480 was derived the previous year.

SW620細(xì)胞The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli.

The cells synthesize only small quantities of carcinoembryonic antigen (CEA), and are highly tumorigenic in nude mice.

The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.

The cells are positive for keratin by immunoperoxidase staining.

The line was derived from a metastasis of the same tumor from which the SW480 (ATCC CCL-228) was derived.

There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.

The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.

N-myc oncogene expression was not detected.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: SW620細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

derived from same individual:ATCC CCL-228

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22545: Lelbovitz A, et al. Detection and analysis of a glucose 6-phosphate dehydrogenase phenotype B cell line contamination. J. Natl. Cancer Inst. 63: 635-645, 1979. PubMed: 288927

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

49853: Witty JP, et al. Modulation of matrilysin levels in colon carcinoma cell lines affects tumorigenicity in vivo. Cancer Res. 54: 4805-4812, 1994. PubMed: 8062282

56129: Gagos S, et al. Chromosomal markers associated with metastasis in two colon cancer cell lines established from the same patient. Anticancer Res. 15: 369-378, 1995. PubMed: 7763008

56130: Melcher R, et al. Spectral karyotyping of the human colon cancer cell lines SW480 and SW620. Cytogenet. Cell Genet. 88: 145-152, 2000. PubMed: 10773689