H9細(xì)胞

細(xì)胞形態(tài)： **樣

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

年限： 53 years

數(shù)量： 大量

ATCC Number： HTB-176?

相關(guān)**： **瘤

Designations: H9 [derivative of HuT 78]

H9細(xì)胞Depositors: RC Gallo, M Popovic

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: lymphoma

Cell Type: cutaneous T lymphocyte;

Cellular Products: interleukin-2 (interleukin 2, IL-2)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:H9細(xì)胞 transfection host (Roche Transfection Reagents)

Receptors: interleukin 2 (IL-2)

Tumorigenic: Yes

Antigen Expression: CD4; HLA A1, B62, C3, DR4, DQ3

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,12

D16S539: 11,12

D5S818: 11

D7S820: 8,11

THO1: 8,9

TPOX: 8,9

vWA: 14,15

Cytogenetic Analysis: This is a near triploid cell line (modal number = 69; range = 58 to 74). The frequency of higher ploidies is 2.5%. The line has an extremely complex karyotype with nearly 60% of the chromosomes in each cell being structurally altered marker chromosomes., Among the markers are t(3p4q), t(5q6q), t(5p6p), i(18q), i(18p); t(4q7p), and del(7)(q32). The first four of these are usually paired. Normal N4, N5, N6, N7, N10, N13, N18, N19, N20 an X are absent.

H9細(xì)胞Isoenzymes: AK-1, 0

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 0

PGM1, 1

PGM3, 0

Age: 53 years

Gender: male

Ethnicity: Caucasian

Comments: The H9 cell line is a clonal derivative of the Hut 78 cell line (see ATCC TIB-161).

The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

H9細(xì)胞Do not allow cell concentration to exceed 3 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC TIB-161

References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

22948: Popovic M, et al. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497-500, 1984. PubMed: 6200935

23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

23367: Popovic M, et al. T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet 2: 1472-1473, 1984. PubMed: 6151082

29139: Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366

32558: Anderson SM, et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189