HCC1500細胞

運輸方式： 凍存運輸

數(shù)量： 大量

組織來源： duct

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CRL-2329?

相關(guān)**： 導管癌

生長狀態(tài)： 貼壁生長

年限： TNM stage IIB, grade 2

器官來源： **

細胞形態(tài)： 上皮樣

HCC1500細胞Designations: HCC1500

Depositors: AF Gazdar, AK Virmani

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIB, grade 2

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 (EGP2)

cytokeratin 19

Permits/Forms: HCC1500細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: February 8, 1995

Applications: HCC1500 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are negative for expression of Her2-neu but positive for expression of p53.

The cells are poorly differentiated.

The cells are positive for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

Receptors: estrogen receptor

progesterone receptor

Oncogene: HCC1500細胞her2/neu -, p53 +

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 10

D16S539: 9,10

D5S818: 11,13

D7S820: 11

THO1: 9

TPOX: 8

vWA: 13,16

Cytogenetic Analysis: near diploid

Age: 32 years

Gender: female

Ethnicity: Black

Comments: This cell line was initiated on 2/8/95 from a patient with a significant family history of early-onset colon cancer as well as a sister with breast cancer. HCC1500細胞The cell line took 14 months to establish.

The tumor was classified as TNM stage IIB, grade 2, invasive ductal carcinoma with 4 out of 24 lymph node metastasis.

The cells are poorly differentiated.

The cells are negative for expression of Her2-neu but positive for expression of p53.

HCC1500 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are positive for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

HCC1500 contains a homozygous deletion at 3p21.3.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: 1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: HCC1500細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 3 to 5 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

38645: Sekido Y, et al. Cloning of a breast cancer homozygous deletion junction narrows the region of search for a 3p21.3 tumor suppressor gene. Oncogene 16: 3151-3157, 1998. PubMed: 9671394