CA46細(xì)胞
細(xì)胞類型： B**細(xì)胞
ATCC Number： CRL-1648?
相關(guān)**： **瘤
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgM; kappa light chain
CA46細(xì)胞Designations: CA46
Depositors: IT Magrath
Isotype: IgM; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted)
Permits/Forms: CA46細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: complement
Antigen Expression: HLA B27 (the patient was HLA A2, A11, B17, B27)
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 11,12
THO1: 7,9
TPOX: 8,9
vWA: 15,16
CA46細(xì)胞Cytogenetic Analysis: 46, XO, XY, dup1(q21-32), t(8;14) (8pter --> 8q23 :: 14q32 --> 14qter; 14pter --> 14q32 :: 8q23 --> 8qter), 13q+, +16
Gender: male
Comments: The cells are EBNA negative and are negative for Fc receptors.
Approximately 12% of the cells express commplement receptors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
A saturation density of 2 X 10 exp6 is obtainable.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 16 hrs
Related Products: CA46細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22548: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
