SJSA-1細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

年限： 19 years *****

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

ATCC Number： CRL-2098?

器官來源： 骨

相關(guān)**： 其他**

SJSA-1細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸

Designations: SJSA-1

Depositors: DN Shapiro

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma; multipotential sarcoma

Permits/Forms: SJSA-1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: 44XY; -1, -4, -7, -9, -11, -14, -15, +del(1)(q32;q42),+5p+, +der(7)t(4;HSR;7)(4qter-4q12::HSR::7p22--7qter), +9p+ and markers; amplified gli sequences were contained in the HSR in derivative No. 7 chromosomes

Age: 19 years *****

Gender: male

Ethnicity: Black

Comments: The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur.

There is amplification of the gene which encodes the p53 associated protein, MDM2.

SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.

Propagation: SJSA-1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Two to three times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22379: Oliner JD, et al. SJSA-1細(xì)胞Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

23076: Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305