T84細(xì)胞

器官來源： 結(jié)腸

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 72 years

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CCL-248?

T84細(xì)胞相關(guān)**： 大腸癌

Designations: T84

Depositors: H Masui

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Disease: colorectal carcinoma

Derived from metastatic site: lung

Cellular Products: T84細(xì)胞carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin

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Applications: transfection host

Receptors: neurotransmitter, expressed

peptide hormone, expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 9

D16S539: 10,11

D5S818: 12

D7S820: 8,10

THO1: 6,9

TPOX: 8,11

vWA: 17,18

Cytogenetic Analysis: T84細(xì)胞The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1-2

PGM1, 1

PGM3, 1

Age: 72 years

Gender: male

Comments: T84細(xì)胞The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice.

The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice.

After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells.

They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport.

This line exhibits tight junctions, and desmosomes between adjacent cells.

The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; fetal bovine serum, 5%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The cells should be maintained at high density (at least 1/4 confluency).

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 1155: Dharmsathaphorn K, et al. A human colonic tumor cell line that maintains vectorial electrolyte transport. Am. J. Physiol. 246: G204-G208, 1984. PubMed: 6141741

26072: Murakami H, Masui H. Hormonal control of human colon carcinoma cell growth in serum-free medium. Proc. Natl. Acad. Sci. USA 77: 3464-3468, 1980. PubMed: 6932031

32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293