MS751細(xì)胞

生長狀態(tài)： 貼壁生長

年限： 47 years

細(xì)胞形態(tài)： 上皮樣

器官來源： 宮頸

ATCC Number： HTB-34?

數(shù)量： 大量

相關(guān)**： 上皮細(xì)胞癌

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: MS751

MS751細(xì)胞Depositors: JA Sykes

Biosafety Level: 2 [Cells contain human papilloma virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cervix

Disease: epidermoid carcinoma

Derived from metastatic site: lymph node

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Isolation: Isolation date: 1974

Tumorigenic: Yes

Antigen Expression: MS751細(xì)胞Blood Type AB; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 12

D16S539: 11

D5S818: 12

D7S820: 9,11

THO1: 6

TPOX: 8

vWA: 16

Cytogenetic Analysis: model number = 39; range = 38 to 41.

This is a hypodiploid human cell line with the modal chromosome number of 39, occurring in 62% of cells. The rate of cells with higher ploidies was 16%. Sixteen markers were found in single copies in all cells. They include del(2) (q34), del(8) (p21), 14p+, der(X)t(X;14) (q28;q13), t(3q8q) and eleven others. MS751細(xì)胞A few different markers, all of which had 11qter--11p13 [der(11)] in common, were found. These markers appeared to be mutually exclusive, each cell having one, and only one, marker at a time. Normal X, N8, N11, N21 and N22 were absent. N1, N10, N15, and N16 were paired, and other normal chromosomes were single.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1-2

Age: 47 years

Gender: female

Ethnicity: Caucasian

Comments: This line was derived by J. Sykes (see also ATCC HTB-33) in 1974.

MS751 cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. [22995] [23180]

More recently, it has been shown that MS751 cells contain a partial HPV-45 genome, and that HPV-45 sequences from the E6/E7 region are expressed as poly(A)+ RNA. [49721]

Propagation: MS751細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22995: Pater MM, Pater A. MS751細(xì)胞Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

49721: Geisbill J, et al. Detection and characterization of human papillomavirus type 45 DNA in the cervical carcinoma cell line MS751. J. Gen. Virol. 78: 655-658, 1997. PubMed: 9049418