A2058細(xì)胞

ATCC Number： CRL-11147?

相關(guān)**： 黑色素瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

器官來源： 皮膚

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

年限： 43 years *****

生長狀態(tài)： 貼壁生長

Designations: A2058

A2058細(xì)胞Depositors: W Stetler-Stevenson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. A2058細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: nerve growth factor (NGF), expressed

laminin, expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,13

D5S818: 9,12

D7S820: 11

THO1: 7,9

TPOX: 8

vWA: 14,18

Age: 43 years *****

Gender: male

Ethnicity: A2058細(xì)胞Caucasian

Comments: This cell line is highly invasive and provides a source of cellular invasion associated proteins (such as the 72000 dalton type IV collagenase.

Tissue inhibitor of metalloproteinase-2 [TIMP-2], autocrine motility factor and the 67000 dalton laminin receptor.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A2058細(xì)胞A subcultivation ratio of 1:6 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22590: Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522

23263: Sherwin SA, et al. Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces. Proc. Natl. Acad. Sci. USA 76: 1288-1292, 1979. PubMed: 375235

23269: Todaro GJ, et al. Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc. Natl. Acad. Sci. USA 77: 5258-5262, 1980. PubMed: 6254071

23404: Stetler-Stevenson WG, et al. The activation of human type IV collagenase proenzyme. Sequence identification of the major conversion product following organomercurial activation. J. Biol. Chem. 264: 1353-1356, 1989. PubMed: 2536363

23549: Stetler-Stevenson WG, et al. Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J. Biol. Chem. 264: 17374-17378, 1989. PubMed: 2793861