NCI-H929細(xì)胞
ATCC Number： CRL-9068?
數(shù)量： 大量
相關(guān)**： 多發(fā)性骨髓瘤
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
年限： 62 years
器官來源： 骨髓
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: NCI-H929 [H929]
NCI-H929細(xì)胞Depositors: National Cancer Institute
Isotype: IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: bone marrow
Disease: plasmacytoma; myeloma
Cell Type: B lymphocyte;
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Receptors: transferrin
Antigen Expression: PCA-1; CD38 +
DNA Profile (STR): NCI-H929細(xì)胞Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15
Cytogenetic Analysis: Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.
Age: 62 years
Gender: female
Ethnicity: Caucasian
Comments: This cell line was established from a malignant effusion in a patient with myeloma.
The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development.
NCI-H929細(xì)胞They are negative for Epstein-Barr virus nuclear antigen (EBNA).
The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA.
There is also an activated ras allele.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 10(5) viable cells/ml.
Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22372: Hollis GF, et al. NCI-H929細(xì)胞Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865
23349: Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157
23351: Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435
