我的性奴的肉玩具1一17心奴,天堂a免费视频在线观看,久久久久久欧美精品无码,26uuu国产精品色噜噜

產(chǎn)品資料

MIA PaCa-2細(xì)胞

如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: MIA PaCa-2細(xì)胞
產(chǎn)品型號: MIA PaCa-2
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

MIA PaCa-2細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。MIA PaCa-2細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


MIA PaCa-2細(xì)胞  的詳細(xì)介紹

MIA PaCa-2細(xì)胞

生長狀態(tài): 貼壁生長

器官來源: 胰腺

ATCC Number: CRL-1420?

相關(guān)**: 腫瘤

年限: 65 years

數(shù)量: 大量

是否是腫瘤細(xì)胞: 1

物種來源: 人

運(yùn)輸方式: 凍存運(yùn)輸

細(xì)胞形態(tài): 其他

Designations: MIA PaCa-2

Depositors: A Yunis

MIA PaCa-2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens

Morphology: attached epithelial with floating rounded cells


Source: Organ: pancreas

Disease: carcinoma

Cellular Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1975

Applications: MIA PaCa-2細(xì)胞The MIA PaCa-2 cell line was established by A. Yunis, et al.

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.

The line is sensitive to asparaginase.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. MIA PaCa-2細(xì)胞A few normal chromosomes are absent.

Isoenzymes: G6PD, B

Age: 65 years

Gender: male

Ethnicity: Caucasian

Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).

The line is sensitive to asparaginase.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: MIA PaCa-2細(xì)胞A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

58034: Yunis AA, et al. MIA PaCa-2細(xì)胞Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918

滬公網(wǎng)安備 31011702004356號

察隅县| 霸州市| 报价| 伊吾县| 武强县| 通州市| 汶上县| 阳信县| 南投县| 宁波市| 施秉县| 阿拉善右旗| 枣强县| 漳州市| 营山县| 安多县| 石景山区| 光泽县| 绥中县| 民权县| 胶南市| 盐边县| 大港区| 龙胜| 石泉县| 留坝县| 普定县| 钟祥市| 嘉善县| 克拉玛依市| 贵定县| 安吉县| 兴义市| 宁津县| 铜鼓县| 延川县| 交口县| 宜宾县| 阿拉尔市| 翁源县| 图木舒克市|