IMR-32細胞

運輸方式： 凍存運輸

細胞形態(tài)： 其他

ATCC Number： CCL-127?

器官來源： 大腦

相關(guān)**： 神經(jīng)母細胞瘤

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

年限： 13 months

數(shù)量： 大量

Designations: IMR-32

Depositors: WW Nichols

IMR-32細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast; neuroblast

Source: Organ: brain

Disease: neuroblastoma

derived from metastatic site: abdominal mass

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Isolation: Isolation date: April, 1967

Applications: transfection host

Virus Resistance: echovirus 11

DNA Profile (STR): IMR-32細胞Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 9

D16S539: 8

D5S818: 11,12

D7S820: 9,10

THO1: 7,9.3

TPOX: 11

vWA: 15

Cytogenetic Analysis: Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively.

Isoenzymes: G6PD, B

Age: 13 months

Gender: male

Ethnicity: Caucasian

Comments: The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. [22190]

The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation.

Two cell types are present.

Predominant is a small neuroblast-like cell.

The other is a large hyaline fibroblast.

IMR-32細胞The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture.

CCL-127 cells may pile up and grow in patches. (Please see the photos of CCL-127 on the ATCC website at www.atcc.org). CCL-127 cells may not become 100% confluent.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Maintain cultures at a cell concentration between 4 X 10 exp4 and 4 X 10 exp5 cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: approximately 20 hrs.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22190: Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762

32287: Rostomily RC, et al. IMR-32細胞Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

32459: Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614