BC-3細胞
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
ATCC Number： CRL-2277?
相關(guān)**： **瘤
年限： 85 years
數(shù)量： 大量
細胞類型： B**細胞
是否是腫瘤細胞： 1
物種來源： 人
細胞形態(tài)： **樣
Designations: BC-3
BC-3細胞Depositors: Cornell Res. Fndn., Inc.
Biosafety Level: 2 [Cells contain Kaposi's sarcoma-associated herpesvirus genome ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: lymphoma
Cell Type: B lymphocyte;
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Applications: transfection host (Roche Transfection Reagents)
Antigen Expression: CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 -; CD5 -, CD8 -; CD19 -; CD20 -; CD21 -; CD22 -
DNA Profile (STR): BC-3細胞Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 14,18
Age: 85 years
Gender: male
Ethnicity: Caucasian
Comments: BC-3 is a primary effusion lymphoma cell line originated in 1995 by Leandros Arvanitakis and Ethel Cesarman.
The cells were established from pleural effusion from a human immunodeficiency virus (HIV) negative patient diagnosed with primary effusion (body cavity based) lymphoma (PEL).
The BC-3 cell line contains the viral genome for Kaposi's sarcoma-associated herpesvirus (KSHV, provisionally designated HHV-8).
BC-3細胞KSHV is a recently identified and partially uncharacterized virus.
BC-3 cells are negative for both HIV and Epstein-Barr virus (EBV).
They are also reported to be negative for herpes simplex virus 1 & 2 (HSV-1 and HSV-2) and cytomegalovirus (CMV).
The BC-3 cell line allows in vitro culture of KSHV viral genomes in the absence of EBV.
It is ideal for the isolation and study of KSHV.
BC-3 has an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate.
The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains) or T-cell lineage-restricted antigens.
The cells do express activation antigens.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23359: Arvanitakis L, et al. BC-3細胞Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood 88: 2648-2654, 1996. PubMed: 8839859
39036: Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999
