MDA-MB-436細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

ATCC Number： HTB-130?

相關(guān)**： 腺癌

細(xì)胞形態(tài)： 其他

年限： 43 years

器官來(lái)源： **

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

Designations: MDA-MB-436

MDA-MB-436細(xì)胞Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: pleomorphic with multinucleated component cells

Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Cellular Products: tubulin; actin

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Tumorigenic: No

DNA Profile (STR): MDA-MB-436細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 10

D16S539: 9,11

D5S818: 13

D7S820: 10

THO1: 9.3

TPOX: 8

vWA: 14,20

Cytogenetic Analysis: modal number = 45

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 43 years

Gender: female

Ethnicity: MDA-MB-436細(xì)胞Caucasian

Comments: The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.

Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22656: Cailleau R, et al. MDA-MB-436細(xì)胞Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903