SK-N-AS細胞
ATCC Number： CRL-2137?
器官來源： 大腦
相關**： 神經(jīng)母細胞瘤
細胞形態(tài)： 上皮樣
細胞類型： 其他細胞類型
運輸方式： 凍存運輸
是否是腫瘤細胞： 1
物種來源： 人
數(shù)量： 大量
年限： 6 years
生長狀態(tài)： 貼壁生長
Designations: SK-N-AS
SK-N-AS細胞Depositors: C Helson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: brain
Disease: neuroblastoma
Derived from metastatic site: bone marrow
Cell Type: neuroblast;
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Isolation: Isolation date: 1981
Receptors: insulin-like growth factor I (IGF-I), expressed
Tumorigenic: SK-N-AS細胞Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
Age: 6 years
Gender: female
Ethnicity: Caucasian
Comments: SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors [Pubmed ID: 2547840]. Retinoic acid partially inhibits proliferation, and cells fail to differentiate [Pubmed ID: 1751405]. The cells exhibit low or no MDR1 expression.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
SK-N-AS細胞O.1 mM Non-Essential Amino Acids (NEAA)
fetal bovine serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 MM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 39 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792
22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824
22444: Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405
22500: El-Badry OM, et al. SK-N-AS細胞Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840
