SCC-4細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 55 years

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CRL-1624?

相關(guān)**： 鱗狀細(xì)胞癌

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來源： 舌頭

運(yùn)輸方式： 凍存運(yùn)輸

Designations: SCC-4

Depositors: JG Rheinwald

SCC-4細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial-like

Source: Organ: tongue

Disease: squamous cell carcinoma

Cellular Products: epidermal keratins; 40 kD keratin

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 11,13

D16S539: 12

D5S818: 13

D7S820: 9,11

THO1: 9.3

TPOX: 8

vWA: 15,17

Age: 55 years

Gender: male

Comments: SCC-4細(xì)胞SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation.

Clonal growth of these cells is improved by using a 3T3 (ATCC CCL-92) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods).

ATCC grows these cells on 56-X, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:

400 ng/ml hydrocortisone

fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Subculture before confluency.

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: SCC-4細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels pre-plated with ATCC 56-X feeder layer (irradiated STO cells).

Incubate cultures at 37C.

Inoculate new flasks at 3 X 10 exp3 cells per sq. cm.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

26113: Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. SCC-4細(xì)胞PubMed: 6160916