SW 962細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

細(xì)胞形態(tài)： 混合型

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： 其他

年限： 64 years

ATCC Number： HTB-118?

相關(guān)**： 腫瘤

Designations: SW962 [SW 962, SW-962]

Depositors: A Leibovitz

SW 962細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixed

Source: Organ: vulva

Disease: carcinoma

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: Blood type A; Rh+

Cytogenetic Analysis: SW 962細(xì)胞hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include: t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: SW 962細(xì)胞The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.

A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. SW 962細(xì)胞PubMed: 327080

22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474