G-292, clone A141B1細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 9 years

ATCC Number： CRL-1423?

相關(guān)**： 骨肉瘤

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 骨

細(xì)胞形態(tài)： 成纖維樣

G-292, clone A141B1細(xì)胞Designations: G-292, clone A141B1

Depositors: PT Peebles

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma

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Receptors: G-292, clone A141B1細(xì)胞epidermal growth factor (EGF)

Isoenzymes: G6PD, B

Age: 9 years

Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: G-292, clone A141B1細(xì)胞24 hours after seeding from a thawed ampule, then 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 22209: . . Pediatr. Res. 12: 485, 1978.

23015: Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. G-292, clone A141B1細(xì)胞PubMed: 7860643