G-292, clone A141B1細胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

是否是腫瘤細胞： 1

物種來源： 人

年限： 9 years

ATCC Number： CRL-1423?

相關(guān)**： 骨肉瘤

運輸方式： 凍存運輸

器官來源： 骨

細胞形態(tài)： 成纖維樣

G-292, clone A141B1細胞Designations: G-292, clone A141B1

Depositors: PT Peebles

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: G-292, clone A141B1細胞epidermal growth factor (EGF)

Isoenzymes: G6PD, B

Age: 9 years

Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: G-292, clone A141B1細胞24 hours after seeding from a thawed ampule, then 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 22209: . . Pediatr. Res. 12: 485, 1978.

23015: Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. G-292, clone A141B1細胞PubMed: 7860643