DMS 114細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
器官來源： 肺
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-2066?
相關(guān)**： 小細(xì)胞肺癌
年限： 68 years
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: DMS 114
Depositors: OS Pettengill, G Sorenson
DMS 114細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
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Receptors: epidermal growth factor (EGF); complement (CR3)
Tumorigenic: Yes
Antigen Expression: Leu 7 +; My23 +; CD11b +
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
DMS 114細(xì)胞D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Age: 68 years
Gender: male
Ethnicity: Caucasian
Comments: The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy.
DMS 114細(xì)胞The cells express HLA class I and class II antigens.
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
23142: Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. DMS 114細(xì)胞PubMed: 6249655
