HCC1419細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

ATCC Number： CRL-2326?

相關(guān)**： 導(dǎo)管癌

運(yùn)輸方式： 凍存運(yùn)輸

年限： TNM stage IIIA, grade 3

器官來(lái)源： **

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: HCC1419

HCC1419細(xì)胞Depositors: AF Gazdar, AK Virmani

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, The line grows as large epithelial attached cells in island-like formation.

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tumor Stage: TNM stage IIIA, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HCC1419細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: December 28, 1994

Applications: The cells overexpress Her2-neu but are negative for expression of p53. HCC1419 is positive for the epithelial cell specific marker epithelial glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

The cells are poorly differentiated.

Receptors: estrogen receptor, negative

progesterone receptor, negative

Oncogene: HCC1419細(xì)胞her2/neu +, p53 -

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 9,12

D16S539: 11,12

D5S818: 10,12

D7S820: 11

THO1: 6,9.3

TPOX: 8

vWA: 16,17

Cytogenetic Analysis: aneuploid

Age: 42 years

Gender: female

Ethnicity: Hispanic

Comments: This cell line was initiated on 12/28/94 from a patient who received prior chemotherapy with no previous family history of breast cancer. The line took 9 months to establish. The tumor was classified as TNM stage IIIA, grade 3, invasive ductal carcinoma with 5 out of 5 lymph node metastasis. HCC1419細(xì)胞The cells are poorly differentiated. The cells overexpress Her2-neu but are negative for expression of p53. HCC1419 is positive for the epithelial cell specific marker epithelial glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: The line grows as large epithelial attached cells in island-like formation.

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771