A-427細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

年限： 52 years

器官來(lái)源： 肺

細(xì)胞形態(tài)： 上皮樣

ATCC Number： HTB-53?

相關(guān)**： 腫瘤

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: A-427

A-427細(xì)胞Depositors: DJ Giard

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

A-427細(xì)胞D13S317: 11,12

D16S539: 11,13

D5S818: 12

D7S820: 8,12

THO1: 9

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker

Isoenzymes: AK-1, 2

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1

Age: 52 years

Gender: male

Ethnicity: Caucasian

Comments: A-427細(xì)胞The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Interval: every 6 to 8 days

Subcultivation Ratio: 1:2 to 1:6

Medium Renewal: Twice per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23218: Giard DJ, et al. A-427細(xì)胞In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758