UM-UC-3細(xì)胞

ATCC Number： CRL-1749?

相關(guān)**： 移行細(xì)胞癌

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

器官來源： 膀胱

運輸方式： 凍存運輸

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

Designations: UM-UC-3

Depositors: HB Grossman

UM-UC-3細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: urinary bladder

Disease: transitional cell carcinoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 8

D16S539: 8,9

D5S818: 12

D7S820: 8,9

THO1: 6,9

UM-UC-3細(xì)胞TPOX: 10

vWA: 17

Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t(1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two to three copies per cell.

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: UM-UC-3細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484

26153: Grossman HB, et al. UM-UC-3細(xì)胞Improved growth of human urothelial carcinoma cell cultures. J. Urol. 136: 953-959, 1986. PubMed: 3761468