NCI-H146細(xì)胞
生長狀態(tài)： 懸浮生長，多細(xì)胞聚集
細(xì)胞形態(tài)： 上皮樣
年限： 59 years
是否是腫瘤細(xì)胞： 1
物種來源： 人
器官來源： 肺
數(shù)量： 大量
ATCC Number： HTB-173?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： 小細(xì)胞肺癌
Designations: NCI-H146 [H146]
NCI-H146細(xì)胞Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Derived from metastatic site: bone marrow
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H146細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1979
Receptors: insulin-like growth factor II (IGF II)
Tumorigenic: Yes
Oncogene: myc +; myb +; raf +; ras +; fms +; fes -
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6,9.3
TPOX: 8,11
vWA: 14,16
Cytogenetic Analysis: This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. NCI-H146細(xì)胞At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1-2
PGM3, 1-2
Age: 59 years
Gender: male
Ethnicity: Caucasian
Comments: The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.
The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified.
The cells do not express vasopressin, oxytocin or gastrin releasing peptide.
There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
Propagation: NCI-H146細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917
22446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
23065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028
23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370
23162: Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893
24389: . . Lung Cancer 4: 155-161, 1988.
