SW837細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
器官來(lái)源： 直腸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
年限： grade IV
ATCC Number： CCL-235?
相關(guān)**： 腺癌
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
Designations: SW837 [SW-837]
SW837細(xì)胞Depositors: A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: rectum
Tumor Stage: grade IV
Disease: adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 523 ng/10 exp6 cells/10 days; keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SW837細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Tumorigenic: Yes
Antigen Expression: HLA A23, A32, B15, B35; blood type O; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16
Isoenzymes: ES-D, 1
G6PD, B
PGD, A
PGM1, 1
PGM3, 1
Age: 53 years
SW837細(xì)胞Gender: male
Ethnicity: Caucasian
Comments: The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.
There is a C -> T mutation in codon 248 of the p53 gene resulting in an Arg -> Trp substitution.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 22147: Chen TR, et al. SW837細(xì)胞Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615
22260: Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989
22930: Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
