Hep 3B2.1-7 (Hep 3B)細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
ATCC Number： HB-8064?
相關(guān)**： 肝癌
器官來(lái)源： 肝
Hep 3B2.1-7 (Hep 3B)細(xì)胞年限： 8 years juvenile
Designations: Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B]
Depositors: Wistar Institute
Biosafety Level: 2 [Cells Contain HEPATITIS B ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: liver
Disease: hepatocellular carcinoma
Cellular Products: alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; [3525]
alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein Hep 3B2.1-7 (Hep 3B)細(xì)胞(alpha-1 acid glycoprotein); [3525]
alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin [3525]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17
Hep 3B2.1-7 (Hep 3B)細(xì)胞Cytogenetic Analysis: modal number = 60 with a subtetraploid mode of 82; has a rearranged chromosome 1 [3525]
Age: 8 years juvenile
Gender: male
Ethnicity: Black
Comments: This line contains an integrated hepatitis B virus genome. [1205] [3525]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1205: Knowles BB, et al.Hep 3B2.1-7 (Hep 3B)細(xì)胞 Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960
3525: Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
22909: Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137
24388: Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851
