SW 1271細(xì)胞

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

年限： 69 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2177?

器官來(lái)源： 肺

相關(guān)**： 小細(xì)胞肺癌

Designations: SW 1271 [SW-1271, SW1271]

SW 1271細(xì)胞Depositors: W McCombs

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1976

Antigen Expression: Blood Type A; Rh +

DNA Profile (STR): SW 1271細(xì)胞Amelogenin: X

CSF1PO: 11

D13S317: 13

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 7,8

TPOX: 8

vWA: 17,20

Age: 69 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: SW 1271細(xì)胞Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53% mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: SW 1271細(xì)胞liquid nitrogen vapor phase