MES-SA/MX2細胞
細胞形態(tài)： 成纖維樣
年限： 56 years
器官來源： **
運輸方式： 凍存運輸
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-2274?
相關(guān)**： 其他**
Designations: MES-SA/MX2
Depositors: WG Harker
MES-SA/MX2細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: uterus
Disease: uterine sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
MES-SA/MX2細胞TPOX: 8,12
vWA: 18
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments: MES-SA/MX2 is a mitoxantrone resistant derivative of the human uterine sarcoma cell line MES-SA (see ATCC CRL-1976).
The line was selected and subcloned in 1988 for resistance to mitoxantrone, an anthracenedione antitumor agent.
These cells were cloned by limiting dilution in soft agar, propagated and tested for sensitively to mitoxantrone.
The clone, designated MES-SA/MX2, was approximately 975 fold less sensitive to mitoxantrone than the parental cells.
The cells display features of both classic multidrug resistance (MDR), P-glycoprotein overexpression and atypical MDR, altered topoisomerase II catalytic activity.
Propagation: MES-SA/MX2細胞ATCC complete growth medium: A 1:1 mixture of Waymouth's MB 752/1 medium and McCoy's 5a medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
These cells grow as a mixture of floating and adherent cells.
Remove the medium with the floating cells, and recover the cells by centrifugation.
Rinse the adherent cells with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach.
MES-SA/MX2細胞Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: parental cell line:ATCC CRL-1976
References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
23058: Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002
