EB1細(xì)胞

年限： 9 years

細(xì)胞形態(tài)： **樣

ATCC Number： HTB-60?

相關(guān)**： **瘤

組織來(lái)源： upper maxilla

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

EB1細(xì)胞Designations: EB1

Depositors: G Trempe, LJ Old

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Tissue: upper maxilla

Disease: Burkitt's lymphoma

Cell Type: B lymphocyte;

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Isoenzymes: ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

Age: EB1細(xì)胞9 years

Gender: female

Ethnicity: Black

Comments: The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.

Viral particles (Epstein-Barr virus) were noted through electron microscopic examination.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Twice per week

Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852

22169: Epstein MA, Barr YM. EB1細(xì)胞Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790

22722: . . Br. J. Cancer 19: 108-115, 1965.

23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014

26323: . . J. Exp. Med. 121: 761-770, 1965.

26324: Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357

26325: Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453

26326: Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. EB1細(xì)胞PubMed: 6818551