CHL-1細胞
數(shù)量： 大量
器官來源： 皮膚
運輸方式： 凍存運輸
細胞形態(tài)： 上皮樣
ATCC Number： CRL-9446?
相關(guān)**： 黑色素瘤
生長狀態(tài)： 貼壁生長
是否是腫瘤細胞： 1
物種來源： 人
Designations: CHL-1
CHL-1細胞Depositors: Berlex Laboratories, Inc.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Cellular Products: human tissue plasminogen activator (t-PA)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: CHL-1細胞transfection host
Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 10(7) cells/ml. [41429]
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 8,11
D16S539: 13
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
Comments: This is a derivative of the RPMI 7932 human melanoma cell line (Bowes).
It produces about 0.1 mU of t-PA per cell per day.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. CHL-1細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 41429: Cashion L, et al. CHL-1細胞Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991
