KATO III細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
生長狀態(tài)： 混合型生長
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 其他
器官來源： 胃
年限： 55 years *****
ATCC Number： HTB-103?
相關(guān)**： 胃癌
KATO III細(xì)胞數(shù)量： 大量
Designations: KATO III
Depositors: M Sekiguchi
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens
Morphology: spherical
Source: Organ: stomach
Disease: KATO III細(xì)胞gastric carcinoma
Derived from metastatic site: pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 7,11
D13S317: 8,12
D16S539: 10,12
D5S818: 10,11
D7S820: 8,12
THO1: 7,9
TPOX: 11
vWA: 14,16
Cytogenetic Analysis: KATO III細(xì)胞The stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Age: 55 years *****
Gender: male
Ethnicity: Asian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: KATO III細(xì)胞Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 xg for 5 to10 minutes.5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.6. Place culture vessels in incubator at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22371: Sekiguchi M, et al. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn. J. Exp. Med. 48: 61-68, 1978. PubMed: 209229
23093: Faust JB, Meeker TC. KATO III細(xì)胞Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
32252: Rieder G, et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128
