Capan-2細胞
細胞形態(tài)： 多邊形
是否是腫瘤細胞： 1
物種來源： 人
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
數(shù)量： 大量
器官來源： 胰腺
年限： 56 years
ATCC Number： HTB-80?
相關**： 腺癌
Designations: Capan-2
Depositors: J Fogh
Capan-2細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: polygonal
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products: mucin (apomucin, MUC-1, MUC-2)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Capan-2細胞Isolation date: 1975
Tumorigenic: Yes
Antigen Expression: Blood Type B; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: modal number = 69; range = 65 to 74.
This is a pseudotriploid human cell line with the modal chromosome number of 69, occurring in 24% of cells. However, cells having chromosome counts from 65 to 70 also occurred at high frequencies. The rate of cells with higher ploidies was 7%. There were eight marker chromosomes common to most cells. Capan-2細胞They included paired del(1) (p22), t(8q11q), i(14q), single i(7p) and four others. Two more markers were seen only in some cells. Only one normal X chromosome was found in G-banded preparation. The Y chromosome was not detected in the Q-banded preparation.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 2
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments: The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Capan-2細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 23102: Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822
