Hs 445細(xì)胞

器官來(lái)源： **結(jié)

ATCC Number： HTB-146?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： **瘤

年限： 56 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

細(xì)胞形態(tài)： **樣

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

Designations: Hs 445

Hs 445細(xì)胞Depositors: RB Owens

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: lymph node

Disease: Hodgkin's disease; Hodgkin's lymphoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,10.3

D13S317: 11,13

Hs 445細(xì)胞D16S539: 9,11

D5S818: 9,12

D7S820: 9,10

THO1: 6,7

TPOX: 8

vWA: 14,17

Isoenzymes: ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1

Age: 56 years

Gender: female

Ethnicity: Caucasian

Comments: Hs 445 was derived in 1970 from an intra-abdominal lymph node of a patient with stage 1 Hodgkin's disease.

By electron microscopy, herpesvirus-like particles have been seen.

0.3% to 0.5% of the cells are positive for EBV early antigen and virion antigen by immunofluorescence.

Propagation: Hs 445細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026

22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Hs 445細(xì)胞Cancer Inst. 65: 715-718, 1980. PubMed: 6932523