SW1116細胞
年限： Dukes' type A, grade III
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CCL-233?
運輸方式： 凍存運輸
數(shù)量： 大量
器官來源： 結(jié)腸
相關(guān)**： 大腸癌
Designations: SW1116 [SW 1116, SW-1116]
Depositors: A Leibovitz
SW1116細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes' type A, grade III
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 2654 ng/10 exp6 cells/10 days; keratin
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Tumorigenic: Yes
Oncogene: myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -
Antigen Expression: blood type O; Rh+
DNA Profile (STR): SW1116細胞Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,14
D16S539: 9,12
D5S818: 11,12
D7S820: 12
THO1: 6
TPOX: 8,11
vWA: 14,19
Cytogenetic Analysis: modal number = 60; range = 50 to 62
The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells.
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Age: 73 years
SW1116細胞Gender: male
Ethnicity: Caucasian
Comments: CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.
N-myc and N-ras oncogene expression were not detected.
Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Cells must be subcultured at about 80% confuency , before they reach 90%.
Protocol: 1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: SW1116細胞1 to 2 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905
