SCC-15細胞
器官來源： 舌頭
ATCC Number： CRL-1623?
相關**： 鱗狀細胞癌
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
是否是腫瘤細胞： 1
物種來源： 人
年限： 55 years
數(shù)量： 大量
Designations: SCC-15
Depositors: JG Rheinwald
SCC-15細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: tongue
Disease: squamous cell carcinoma
Cellular Products: epidermal keratins; 40 kD keratin; low levels of involucrin
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,14
D16S539: 12,15
SCC-15細胞D5S818: 12
D7S820: 10,11
THO1: 9,9.3
TPOX: 8
vWA: 15,17
Age: 55 years
Gender: male
Comments: Unlike SCC-4 (ATCC CRL-1624), this line does not grow well in semi-solid medium, and does not require a feeder layer.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: SCC-15細胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336
26113: Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916
26114: Wu YJ, Rheinwald JG. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell 25: 627-635, 1981. SCC-15細胞PubMed: 6169443
