LS411N細(xì)胞

數(shù)量： 大量

ATCC Number： CRL-2159?

相關(guān)**： 大腸癌

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 盲腸

細(xì)胞形態(tài)： 上皮樣

年限： Dukes' type B

運(yùn)輸方式： 凍存運(yùn)輸

Designations: LS411N

Depositors: L Suardet

LS411N細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cecum

Tumor Stage: Dukes' type B

Disease: colorectal carcinoma

Cellular Products: transforming growth factor beta 1 (TGF, 189 pg per 10 exp6 cells per 24 hours)

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Isolation: Isolation date: 1985

Tumorigenic: Yes

Antigen Expression: carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative

Cytogenetic Analysis: LS411N細(xì)胞modal number = 75, X.

Age: 32 years

Gender: male

Ethnicity: Caucasian

Comments: LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes' B, poorly differentiated cecal carcinoma.

The LS411 cell line was found to be contaminated with mycoplasma, and was passed through nude mice to clear the cells of mycoplasma.

The cells obtained after passage in nude mice are referred to as LS411N cells.

A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline.

Approximately 20% of LS411N cells express surface carcinoembryonic antigen (CEA).

The cells express low levels of ICAM-1 HLA class I antigen beta-2-microglobulin.

The cells secrete low levels of latent TGF-beta 1.

TGF-beta 1 and TGF beta?2 are do not inhibit the proliferation of LS411N cells.

LS411N細(xì)胞Colony forming efficiency was 25% in methylcellulose medium.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 24 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23099: Suardet L, et al. LS411N細(xì)胞Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643