SW 1783細(xì)胞

器官來(lái)源： 大腦

年限： grade III

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： HTB-13?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

細(xì)胞形態(tài)： 成纖維樣

Designations: SW 1783 [SW-1783, SW1783]

Depositors: A Leibovitz

SW 1783細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: brain

Tumor Stage: grade III

Disease: astrocytoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Temple Texas, United States

Isolation date: 1977

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,13

SW 1783細(xì)胞D13S317: 9,11

D16S539: 12

D5S818: 12,13

D7S820: 9,13

THO1: 6,8

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1

Age: SW 1783細(xì)胞68 years *****

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Interval: every 6 to 8 days

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

References: 32274: Koochekpour S, et al. SW 1783細(xì)胞Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765