MNNG/HOS (Cl #5)細(xì)胞

年限： 13 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 骨

ATCC Number： CRL-1547?

相關(guān)**： 骨肉瘤

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: MNNG/HOS Cl #5 [R-1059-D]

Depositors: JS Rhim

MNNG/HOS (Cl #5)細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: bone

Disease: osteosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

MNNG/HOS (Cl #5)細(xì)胞D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 8,11

vWA: 18

Isoenzymes: G6PD, B

Age: 13 years

Gender: female

Ethnicity: Caucasian

Comments: This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.

Propagation: MNNG/HOS (Cl #5)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.

References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046

26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298

32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. MNNG/HOS (Cl #5)細(xì)胞Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224