HuT 102細(xì)胞

年限： 26 years

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 1

物種來源： 人

ATCC Number： TIB-162?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 其他**

生長狀態(tài)： 懸浮生長，多細(xì)胞聚集

Designations: HuT 102

Depositors: AF Gazdar

HuT 102細(xì)胞Biosafety Level: 2 [Cells Contain RETROVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: lymphoma; mycosis fungoides

Cell Type: cutaneous T lymphocyte;

Cellular Products: HTLV-I (human T cell leukemia virus)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: interleukin 2 (IL-2)

Antigen Expression: CD4; Homo sapiens

DNA Profile (STR): HuT 102細(xì)胞Amelogenin: X,Y

CSF1PO: 8,11

D13S317: 11,13

D16S539: 12

D5S818: 8,13

D7S820: 8,10

THO1: 7,8

TPOX: 6

vWA: 16,19

Age: 26 years

Gender: male

Ethnicity: Black

Comments: The Hut 102 cell line was derived from the peripheral blood of a 26 year old Black male patient with mycosis fungoides.

This line has the properties of a mature human T cell with helper/inducer activity.

It has receptors for IL-2, and the growth rate is stimulated by IL-2.

The line is E-rosette positive (E+), and negative for Epstein-Barr virus nuclear antigen (EBNA-), surface immunoglobulin (sIg-) and terminal deoxynucleotidyl transferase (TdT-).

HuT 102細(xì)胞The cells release a type C retrovirus that has been associated with human T cell lymphomas and should be handled as a biohazard.

The line was originally thought to produce IL-2; however, recent studies have failed to detect IL-2 mRNA production in Hut 102 cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.Maintain cell density between 2 X 10 exp5 and 8 X 10 exp5 viable cells/ml. Do not allow density to exceed 1 X 10 exp6 cells/ml. Background debris is often present.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: about 41 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

23184: Hsu SM, et al. Expression of p55 (Tac) interleukin-2 receptor (IL-2R), but not p75 IL- 2R, in cultured H-RS cells and H-RS cells in tissues. Am. J. Pathol. 136: 735-744, 1990. PubMed: 1691591

23271: Poiesz BJ, et al. HuT 102細(xì)胞Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc. Natl. Acad. Sci. USA 77: 7415-7419, 1980. PubMed: 6261256

23311: Wano Y, et al. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc. Natl. Acad. Sci. USA 85: 9733-9737, 1988. PubMed: 3059351