HCC1954細(xì)胞

器官來源： **

組織來源： duct

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

年限： TNM stage IIA, grade 3

是否是腫瘤細(xì)胞： 1

物種來源： 人

ATCC Number： CRL-2338?

相關(guān)**： 導(dǎo)管癌

細(xì)胞形態(tài)： 其他

Designations: HCC1954

Depositors: AF Gazdar, AK Virmani

HCC1954細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: large epithelial cells with occasional vacuoles

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIA, grade 3

Disease: ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. HCC1954細(xì)胞They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: October 30, 1995

Applications: HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.

Receptors: estrogen receptor, not expressed

progesterone receptor, not expressed

Oncogene: her2/neu + (overexpressed)

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 8,9

D16S539: 9,11

D5S818: 11

D7S820: 10,11

THO1: 6,7

TPOX: 8,9

vWA: 18,19

HCC1954細(xì)胞Age: 61 years *****

Gender: female

Ethnicity: East Indian

Comments: The HCC1954 is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish.

HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.

HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

Her2/neu is overexpressed in the ELISA assay.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: HCC1954細(xì)胞A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

normal (or near-normal) cell line established from the same patient:ATCC CRL-2339

purified DNA:ATCC CRL-2338D

purified RNA:ATCC CRL-2338R

cell pellet:ATCC CRL-2338P

References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771