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HEP G2/2.2.1細胞

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產品名稱: HEP G2/2.2.1細胞
產品型號: HEP G2/2.2.1
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

HEP G2/2.2.1細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環(huán)境、與品質良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。HEP G2/2.2.1細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


HEP G2/2.2.1細胞  的詳細介紹

HEP G2/2.2.1細胞

數(shù)量: 大量

ATCC Number: CRL-11997?

相關**: 肝癌

是否是腫瘤細胞: 1

物種來源: 人

生長狀態(tài): 貼壁生長

器官來源: 肝

年限: 15 years

運輸方式: 凍存運輸

細胞形態(tài): 上皮樣

Designations: HEP G2/2.2.1

HEP G2/2.2.1細胞Depositors: Northeastern Ohio University College of Medicine

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial


Source: Organ: liver

Disease: hepatocellular carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 9,13

D16S539: 12,13

HEP G2/2.2.1細胞D5S818: 11,12

D7S820: 10

THO1: 9

TPOX: 8,9

vWA: 17

Age: 15 years

Gender: male

Ethnicity: Caucasian

Comments: Cell line was derived from the hepatocellular carcinoma cell line, HepG2 (ATCC HB-8065). The parental cells were stably transfected at passage 48 with a human cholesterol 7 alpha-hydroxylase (CYP7) minigene/Luciferase construct.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate and 1200 mg/L sodium bicarbonate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: HEP G2/2.2.1細胞These cells are slow to attach after subculture. Allow 4 to 5 days for reattachment.

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.


Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

parental cell line:ATCC HB-8065

References: 40153: Chiang JY, Stroup D. HEP G2/2.2.1細胞Assay for agents that affect cholesterol 7alpha-hydroxylase expression and a characterization of its regulatory elements. US Patent 5,821,057 dated Oct 13 1998

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