HCC1395細(xì)胞

器官來(lái)源： **

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2324?

相關(guān)**： 導(dǎo)管癌

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

組織來(lái)源： duct

年限： TNM stage I, grade 3

數(shù)量： 大量

HCC1395細(xì)胞Designations: HCC1395

Depositors: AF Gazdar, AK Virmani

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage I, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 (EGP2)

cytokeratin 19

Permits/Forms: HCC1395細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: December 14, 1994

Applications: The cells are poorly differentiated and vacuolated.

The cells are negative for expression of Her2-neu but positive for expression of p53.

HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -).

Receptors: estrogen receptor, negative according to depositor

progesterone receptor, negative

Oncogene: her2/neu -, p53 +

DNA Profile (STR): HCC1395細(xì)胞Amelogenin: X

CSF1PO: 11

D13S317: 9

D16S539: 11

D5S818: 11

D7S820: 10,12

THO1: 9.3

TPOX: 11

vWA: 15

Cytogenetic Analysis: polyploid

Age: 43 years *****

Gender: female

Ethnicity: Caucasian, White

Comments: This cell line was initiated on 12/14/94 from a patient with a family history of cancer (patient's mother had breast cancer).

The cell line took 14 months to establish.

The tumor was classified as TNM stage I, grade 3, invasive ductal carcinoma with 0 out of 34 lymph node metastasis.

The cells are poorly differentiated and vacuolated.

The cells are negative for expression of Her2-neu but positive for expression of p53.

HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.

The cells are negative for expression of estrogen receptors (ER -) according to depositor and negative for expression of progesterone receptors (PR -).

An EBV transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325.

Propagation: HCC1395細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

purified DNA:ATCC CRL-2324D

normal (or near-normal) cell line established from the same patient:ATCC CRL-2325

purified RNA:ATCC CRL-2324R

cell pellet:ATCC CRL-2324P

References: 38266: Gazdar AF, et al. HCC1395細(xì)胞Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771