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產(chǎn)品資料

MDA-MB-415細(xì)胞

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產(chǎn)品名稱: MDA-MB-415細(xì)胞
產(chǎn)品型號(hào): MDA-MB-415
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

MDA-MB-415細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。MDA-MB-415細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


MDA-MB-415細(xì)胞  的詳細(xì)介紹

MDA-MB-415細(xì)胞

ATCC Number: HTB-128?

相關(guān)**: 腺癌

年限: 38 years *****

運(yùn)輸方式: 凍存運(yùn)輸

器官來源: **

是否是腫瘤細(xì)胞: 1

物種來源: 人

細(xì)胞形態(tài): 上皮樣

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

數(shù)量: 大量

Designations: MDA-MB-415

Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: MDA-MB-415細(xì)胞See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1975

Tumorigenic: No

Antigen Expression: Blood Type O

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11,13

D16S539: 13

MDA-MB-415細(xì)胞D5S818: 11,13

D7S820: 9,10

THO1: 7,9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations.

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, A

GLO-I, 1-2

Me-2, 2

PGM1, 1

PGM3, 1

Age: 38 years *****

Gender: female

Comments: MDA-MB-415細(xì)胞The cells express the WNT7B oncogene [PubMed: 8168088].

The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.

The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization.

Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

purified DNA:ATCC 45524

purified DNA:ATCC 45525

purified DNA:ATCC HTB-128D

References: 1206: Brinkley BR, et al. MDA-MB-415細(xì)胞Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22329: Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088

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