MDA-MB-415細(xì)胞

ATCC Number： HTB-128?

相關(guān)**： 腺癌

年限： 38 years *****

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： **

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

Designations: MDA-MB-415

Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: MDA-MB-415細(xì)胞See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

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Isolation: Isolation date: 1975

Tumorigenic: No

Antigen Expression: Blood Type O

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11,13

D16S539: 13

MDA-MB-415細(xì)胞D5S818: 11,13

D7S820: 9,10

THO1: 7,9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: modal number =73; range = 65 to 76. The stemline chromosome number is hypertriploid with the 2S component occurring at 0.8%. Ten markers (del(2), t(6;?), t(6;?), t(2;12), t(14;?), and single or paired M6, M7, M8, M9, and M10) were common to all cells, and four others (M13, M15, M16, and t(13,21) occurred in some cells. The X chromosome was generally tetrasomic and chromosome 4 was tetrasomic or pentasomic (P35). No Y chromosomes were detected in QM stained preparations.

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, A

GLO-I, 1-2

Me-2, 2

PGM1, 1

PGM3, 1

Age: 38 years *****

Gender: female

Comments: MDA-MB-415細(xì)胞The cells express the WNT7B oncogene [PubMed: 8168088].

The patient presenting with the tumor was from Paraguay and, although listed as Caucasian, may have had mixed ancestry as suggested by the presence of G6PD type A phenotype in the cells.

The line forms flat, spreading plaques of epithelia which exhibit desmosomes, extensive microtubules and microfilaments upon examination with the electron microscope. It is not amenable to trypsinization.

Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine supplemented with 10 mcg/ml insulin and 10 mcg/ml glutathione, 85%; fetal bovine serum, 15%

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

purified DNA:ATCC 45524

purified DNA:ATCC 45525

purified DNA:ATCC HTB-128D

References: 1206: Brinkley BR, et al. MDA-MB-415細(xì)胞Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22329: Beall PT, et al. Polypeptides that inhibit human breast cancer cell division. Cancer Biochem. Biophys. 3: 93-96, 1979. PubMed: 552902

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088