NCI-N87細(xì)胞

ATCC Number： CRL-5822?

運輸方式： 凍存運輸

相關(guān)**： 胃癌

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

器官來源： 胃

細(xì)胞形態(tài)： 上皮樣

Designations: NCI-N87 [N87]

Depositors: J Park

NCI-N87細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: stomach

Disease: gastric carcinoma

Derived from metastatic site: liver

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Isolation: Isolation date: 1976

Receptors: acetylcholine, muscarinic, expressed [23078]

Tumorigenic: Yes

Oncogene: myc +; erb B2 +

DNA Profile (STR): NCI-N87細(xì)胞Amelogenin: X,Y

CSF1PO: 8,12

D13S317: 8,11

D16S539: 9,13

D5S818: 12,13

D7S820: 10,11

THO1: 9

TPOX: 9,11

vWA: 15,16

Cytogenetic Analysis: near diploid; DM were present in 64% of cells examined

Gender: male

Comments: NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were fiound to express receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. NCI-N87細(xì)胞The cell line expressed levels of c-myc and c-erb-B 2 RNA that were comparable to other cell lines.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: They grow as an adherent monolayer of tightly knit epithelial cells.

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: NCI-N87細(xì)胞A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 47 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23078: Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.