LS 180細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
年限： Dukes' type B
ATCC Number： CL-187?
器官來(lái)源： 結(jié)腸
相關(guān)**： 大腸癌
細(xì)胞形態(tài)： 上皮樣
Designations: LS 180
Depositors: Northwestern University
LS 180細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes' type B
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen
interleukin 10
interleukin 6
mucin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LS 180細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Antigen Expression: serologically defined colon cancer antigen 3; Homo sapiens, expressed
HLA A2, B13, B50; Blood type O
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,13
D13S317: 10
D16S539: 11,13
D5S818: 10,15
D7S820: 11,9.3
THO1: 6,7
TPOX: 8,9
vWA: 15,18
Cytogenetic Analysis: modal number = 45; range = 42 to 47.
LS 180細(xì)胞The stemline karyotype was 45,t(X;5) (q12;q31) with tetraploid-like cells occurring at 2.2 percent. Occasional changes in karyotypes were also noted as a result of balanced translocations producing new marker chromosomes. In conjunction with this, decreases in chromosomes involving interchromosomal exchanges were seen. The karyotype was exactly the same as that originally described by the depositor.
Isoenzymes: ADA, 1
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Age: 58 years
Gender: female
Ethnicity: Caucasian
Comments: This line has never been trypsinized; however, the variant LS 174T (ATCC CL-188) has been subcultured by trypsinization.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove most medium. Dislodge cells from the floor of the flask by banging flask, washing cells by repeat pipetting and scraping gently. LS 180細(xì)胞Aspirate and dispense into new flasks. These cells have never been trypsinized.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Two to three times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derived from same individual:ATCC CL-188
References: 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041
3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980
22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615
22511: Gastl GA, et al. LS 180細(xì)胞Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
32532: Schuetz EG, et al. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc. Natl. Acad. Sci. USA 93: 4001-4005, 1996. PubMed: 8633005
