NCI-H1651細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

年限： 71 years

ATCC Number： CRL-5884?

數(shù)量： 大量

相關(guān)**： 非小細(xì)胞肺癌

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 肺

Designations: NCI-H1651 [H1651]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: NCI-H1651細(xì)胞See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: adenocarcinoma; non-small cell lung cancer

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): NCI-H1651細(xì)胞Amelogenin: X

CSF1PO: 10,13

D13S317: 11

D16S539: 11

D5S818: 12,14

D7S820: 8

THO1: 8

TPOX: 11

vWA: 18

Age: 71 years

Gender: male

Comments: The line was established in May 1987.

The tissue donor was a non-smoker.

Propagation: ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS

The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin

0.01 mg/ml transferrin

25 nM sodium selenite

50 nM Hydrocortisone

1 ng/ml Epidermal Growth Factor (do not filter)

0.01 mM ethanolamine

NCI-H1651細(xì)胞0.01 mM phosphorylethanolamine

100 pM triiodothyronine

0.5% (w/v) bovine serum albumin

10 mM HEPES

0.5 mM sodium pyruvate

2mM L-glutamine(for final conc. of 4.5 mM)

10% FBS

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin- 0.53mM EDTA solution. Remove the solution and add an additional 2 to 3 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.4. Add 6.0 to 8.0 ml of complete growth medium containing 10% FBS and aspirate cells by gently pipetting4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipettingCentrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new culture vessels.

Subcultivation Ratio: A subcultivation of 1:3 to 1:8 is recommended

Medium Renewal: Twice weekly

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

Medium additive:ATCC 30-2214

References: 23570: . NCI-H1651細(xì)胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996