BT-483細(xì)胞

年限： 23 years

細(xì)胞形態(tài)： 上皮樣

器官來(lái)源： **

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： HTB-121?

相關(guān)**： 導(dǎo)管癌

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

BT-483細(xì)胞Designations: BT-483

Depositors: EY Lasfargues

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Disease: ductal carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. BT-483細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,12

D13S317: 8,12

D16S539: 11,12

D5S818: 11

D7S820: 9,10

THO1: 5,9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number = 72; range = 46 to 130

Isoenzymes: AK-1, 1

BT-483細(xì)胞ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 2

PGM3, 2

Age: 23 years

Gender: female

Ethnicity: Caucasian

Comments: The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: BT-483細(xì)胞Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

purified DNA:ATCC HTB-121D

References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

23093: Faust JB, Meeker TC. BT-483細(xì)胞Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216