HCC38細(xì)胞

ATCC Number： CRL-2314?

相關(guān)**： 導(dǎo)管癌

年限： TNM stage IIB, grade 3

組織來源： duct

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

器官來源： **

數(shù)量： 大量

Designations: HCC38

Depositors: AF Gazdar, AK Virmani

HCC38細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Tumor Stage: TNM stage IIB, grade 3

Disease: primary ductal carcinoma

Cellular Products: Epithelial glycoprotein 2 [EGP2]; cytokeratin 19

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Restrictions: HCC38細(xì)胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

Isolation: Isolation date: April 27, 1992

Applications: HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

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The cells are negative for expression of Her2-neu but positive for expression of p53

Receptors: estrogen receptor, not expressed

progesterone receptor, not expressed

Oncogene: her2/neu -; p53 +

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

HCC38細(xì)胞D13S317: 12,14

D16S539: 10,14

D5S818: 9

D7S820: 10

THO1: 9.3

TPOX: 9,12

vWA: 16,17

Cytogenetic Analysis: Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%

Age: 50 years *****

Gender: female

Ethnicity: Caucasian, White

Comments: The cells are negative for expression of Her2-neu but positive for expression of p53

This cell line was initiated on 4/27/92 and took 32 months to establish.

HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer.

The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.

HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

HCC38 contains a homozygous deletion at 3p12.

Cytogenetics: Derivatives and Markers

At least 45 distinct derivative chromosomes were detected in most metaphases, including two large metacentric markers which are approximately 1.5 times longer than a normal A group chromosome.

Other derivative chromosomes: del(1)(q24); del(1)(p22); del(1)(p13); add(1)(p12); del(2)(p16); add(3)(q10); del(3)(q13); ?del(4)(q13) (two copies per cell); del(5)HCC38細(xì)胞(q23q33); der (7)(pter?q11::?hsr); del(7)(p?);

del(9)(p12); add(9)(p10); ?der(11); add(12)(q24)(very long); add(14)(p11); add(17)(p12); der(18); der(X); plus approx. 17-24 markers of unknown origin per cell.

Cytogenetics: Comments

This is a hyper-triploid human cell line with a modal chromosome number of 75.

Homogeneously staining regions and dicentric chromosomes were observed. Every chromosome pair had a least one rearrangement; however, normal copies of chromosomes 2, 6, 11, 13, 16 and 20 were consistently seen.

No normal X chromosomes were observed and Y chromosomes were absent by QM staining.

The following structural rearrangements were observed in 30 metaphases: an acentric fragment in 2/30 metaphases, a minute in 3/30, a chromosome break in 3/30, a chromatid break in 5/30, a ring chromosome in 1/30, and double minutes in 11/30 (1-5 copies).

Pulverized chromosomes were reported in 5% of metaphases. C-banding revealed that several of the large markers are dicentric. No normal X chromosomes were observed and Y chromosomes were absent by QM staining.

Normal copies of chromosomes 2,6,11,13,16 and 20 were seen.

Composite karyotype: 65-79<3n> der(X), -1,-1,-1, del(1)(q24), del(1)(p22),del(1)(p13),add(1)(p12),-2,del(2)(p16)x2,-3,-3,-3, ?add(3)(q10),del(3)(q13)x2,-4,-4,-4, HCC38細(xì)胞del(4)(q13)x2,-5, -5,-5,del(5)?(q23q33)x2,-6,-6,-7,-7,-7,

der (7)(pter?q11::?hsr), del(7)(p?)x2,-8,-8,-8,-9,-9,-9,del(9)(p12)x2,add(9)(p10)x2, -10,-10,-10,-11,?der(11),-12,-12,-12, add(12)(q24), -13,-13,-14,-14,-14,

add(14)(p11)x2,-15,-15,-15,-17,-17,-17, add(17)(p12)x2,-18,-18,-18,der(18),-19,-19,-19,-21,-21, -22,-22,+17 to 24 mar[cp11].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 3 to 5 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

EBV-transformed cell line from the same patient:ATCC CRL-2346

purified DNA:ATCC CRL-2314D

purified RNA:ATCC CRL-2314R

References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

38330: Sundarsan V, et al. Homozygous deletions at 3p12 in breast and lung cancer. Oncogene 17: 1723-1729, 1998. PubMed: 9796701